Abstract for my work
Primordial germ cell (PGC) is a group of embryonic cells that will develop into mature gametes in the early stages of development. Buckyball is a factor found in fish that plays an important role in germ plasma assembly. However, the effects of its tissue-specific overexpression in primordial germ cells (PGC) remains unclear. This project used the unique Gal4-UAS expression system to ensure PGC-specific overexpression of Buckyball, combined with the strong advantages of the China zebrafish resource center(CZRC), to study the effect of buckyball overexpression on the number of PGCs and gonadal development at DNA level. Unlike temporary gene knockouts at RNA level, changes in DNA sequence allows us to observe the sustained effects of buckyball genes on following PGC differentiation. We have found some intriguing facts and the work is still going on.
Reference
Asakawa K, Abe G, Kawakami K. Cellular dissection of the spinal cord motor column by BAC transgenesis and gene trapping in zebrafish[J]. Frontiers in neural circuits, 2013, 7: 100.